|
 |
Sheng
Wei, M.D.
Associate Professor in the Department of Oncologic Sciences
Member-in-Residence
of the Moffitt Cancer Center
E-mail: Sheng.Wei@moffitt.org
Phone: 813-745-3934 |
Training
M.D.: Tianjin Medical University, Tianjin, China.
Postdoctoral Fellow: Univ. of South Florida, Department of Medical Microbiology
and Immunology.
Research
Interests
Dr. Wei's laboratory is focused on elucidation of the signal pathway for
activation of human neutrophil function by cytokines and bacterial products.
He has made a key discovery that GM-CSF, IL-2 and LPS signal via src-kinase-related
protein tyrosine kinase, Lyn, suggesting that Lyn is a common element
in neutrophil activation. Lyn play a key role in neutrophil survival induced
by GM-CSF. Use of anti-sense oligonucleotides to Lyn indicated that anti-sense
Lyn could readily block GM-CSF induced neutrophil survival. Dr. Wei also
identified a key role for MAP kinase in internalization and growth inhibition
of C. albicans using the MAP kinase inhibitor and dominant negative MEK
expression. An important discovery was that MAP kinase regulated the migration
of proteolytic granules to the point of contact with C. albicans within
neutrophil. Another focus is on the signals that control of Natural Killer
Cell (NK) mediated tumor lysis. Analysis of signal molecules indicated
that target ligation triggers a Ras-independent MAP kinase pathway that
is required for lysis of the ligated tumor cells. Target engagement caused
NK cells to rapidly activate MAP kinase and perforin/granzyme B polarization
within NK cells towards the contacted target cells. The present effort
is to further define downstream event from MAP kinase that could mediated
functional activation of both human neutrophil and
NK cells.
Search
for publications by: 
This
search will be conducted at the US National Library of Medicine (NLM) and PubMed.
Selected
Publications
1. Wei, S., D. Gilvary, B. Corliss, S. Sebti, J. Sun, D. Straus, P. Leibson,
J. Trapani, A. Hamilton, M. Weber and JY. Djeu. 2000. Direct tumor lysis
by natural killer cells utilizes a Ras-independent MAPK signal pathway.
J. Immunol. 165: 3811.
2. Wei,
S., J. H. Liu, P. K. Burnette, A. M. Gamero, M. E. Elkabani, J. Y. Djeu.
2000. IL-2 modulates lyn and MAP protein kinase activity in human neutrophils.
Immunobiology (in press).
3. K. Jiang,
B. Zhong, D. Gilvary, B. Corliss, E. Hong-Geller, S. Wei and J.Y. Djeu.
2000. Pivotal role of PI-3 kinase in regulation of cytotoxicity of Natural
Killer Cells. Nature Immunology (in press).
4. J. H.,
S. Wei, T. Lamy, P. K. Epling-Burnette, G. Starkebaum, J. Y. Djeu, T.
P. Loughran. 2000. Chronic neutropenia mediated by FAS ligand. Blood 95:3219.
|
